Full Name: Human Alanine Aminotransferase Recombinant from human
The DNA encoding Human ALT is cloned from the human liver cDNA library, harboring the same amino acid sequence as the native cytosolic ALT ananlyzed by Ishiguro er al. r-hALT is expressed as an active holoenzyme in E. coli. r-hALT is purified to homogeneity as judged by SDS-PAGE. r-hALT is indistinguishable from native one in terms of molecular and catalytic properties.
1) Molecular size is 55 kDa on SDS-PAGE and 101 kDa on gel filtration, indicating a homodimer of two identical subunits.
2) Isoelectric Point is 6.5 when measured by isoelectric focusing.
3) Km value is measured to be 20.5 mM for L-Ala, and 0.443 mM for 2-oxoglutarate.
4) r-hALT shows high compatibility to native ALT on serum ALT assay in clinical chemistry tests.