Full Name: Leukemia Inhibitory Factor, recombinant from human
Host cell: E.coli
Molecular weight: 20 kDa
Purity: ≧95% by SDS-PAGE analysis
Endotoxin:
Concentration:
Lyophilized
Lyophilized from a 0.22 mm filtered solution of PBS+1 M NaCl+1 mM EDTA.
It is recommended that reconstitute in purified water to a concentration of 0.1-1.0 mg/mL.
Biological activity: Measured by alkaline phosphatase activity
Condition for Storage:
Store below -20℃
After reconstitute, store at -70 ℃. Avoid repeated freeze / thaw cycles
References:
1. Hilton D. and Gough M. (1998). Cytokines, chapter 20, 277-288. Academic Press Limited.
2. Miyaoka Y. Tanaka M, Naiki T, Miyajima A(2006). J Biol Chem.281(49):37913-20.
3. Laurence Dahéron1, Sarah L. Opitz1, et al. (2004)STEM CELL.S22(5):770–778
4. Jessica C Hogan and Jacqueline M Stephens(2005) J Endocrinol.185,485-496
5. Stephens JM,Lumpkin SJ et al.(1998) J Biol Chem.;273(47):31408-16.
6. Gafni O, Weinberger L et al.(2013) Nature;504(7479):282-6.