Full Name β-Nicotinamide-Adenine Dinucleotide, Oxidized Form (NAD)
Formula Weight: 743.41 (as anhydrous free acid)
841.42 (NADP+.Na2.3H2O)
Formula: C21H28N7O17P3
Purity: ≧ 93% when determined by enzymatic analysis with glucose-6-phosphate dehydrogenase at pH 7.5
*G6PDH = Glucose-6-phosphate dehydrogenase (yeast)(EC 1.1.1.49.)
Sodium Content: 3.0 ± 1.5% by atomic absorption spectrophotometry
Water Content: < 8% by Karl Fisher method
Spectral Analysis:
1. ε at 260 nm and pH7.5: (18.0 ± 0.8) x 103L.mole-1.cm-1
2. ε when reduced with G6PDH* at 340nm and pH7.5:
(6.2 ± 0.3) x 103L.mole-1.cm-1
3. Ratios at pH 7.5: A250/A260 (0.83 ± 0.03)
A280/A260 (0.21 ± 0.02)
4. Ratio when reduced with G6PDH* at pH 7.5:
A340/A260 (0.43 ± 0.02)
Condition for Storage:
Keep tightly stoppered in the dark below 5°C.
Moisture will reduce purity.
For prolonged storage, keep below -20°C.
Shipping Condition: Refrigerate