Full Name: Glutamate Dehydrogenase (Yeast) (GLDH)
Specifications
L-Glutamate: NADP+ oxidoreductase (deaminating) (EC 1.4.1.4) from yeast
Preparation: Lyophilized
Reaction Equation:
L-Glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH
Specific activity: > 10 U/mg protein
(for reduction of α-ketoglutarate to L-glutamate at 25°C and pH 7.6)
Contaminants: (as GlDH activity = 100%)
Glucose-6-phosphate dehydrogenase < 0.1 %
Phosphogluconate dehydrogenase < 0.5 %
Glutamate dehydrogenase (NAD+) < 0.1 %
Glutathione reductase < 0.1 %
NADPH oxidase < 0.01 %
State: Ammonium sulfate free, lyophilized powder of highly purified enzyme
One mg of powder contains the following
Protein 700 ± 200 µg
K-PO4 300 ± 300 µg
Condition for Storage: Lyophilized at -20°C.