Full Name: Glucose-6-Phosphate Dehydrogenase (Yeast) (G6PDH (Y) Solution)
Specifications
D-Glucose-6-Phosphate: NADP+ 1-oxidoreductase (EC 1.1.1.49) from yeast
Standard: G-6-PDH-52
Specific Activity: > 250 units/mg protein (at 25°C and pH 8.5)
Preparation: Ammonium Sulfate
Reaction Equation:
D-Glucose-6-Phosphate + NADP+ = D-glucono-1,5-lactone-6-phosphate + NADPH + H+
Contaminants: (as G-6-PDH activity = 100%)
Gluutathione reductase < 0.2 %
Hexokinase < 0.02 %
Phosphogluconate dehydrogenase < 0.01 %
Phosphoglucose isomerase < 0.01 %
Phosphoglucomutase < 0.01 %
Creatine kinase < 0.001 %
Myokinase < 0.01 %
ATPase < 0.001 %
State: Purified enzyme in solution
One mL of suspension contains the following
Protein 0.5 ~ 20 mg
K-PO4 0.01 ~ 0.1 mol/L
(NH4)2SO4 3.3 mol/L
EDTA¥Na2 0.1 ~ 5.0 mmol/L
pH 7.0 ± 0.5
Conditions for Storage: 2 – 10 °C