Full Time: Pyruvate Kinase (Rabbit Muscle)
Specifications
ATP: pyruvate 2-ortho-phosphotransferase (EC 2.7.1.40) from rabbit muscle
Specific activity: > 200 U/mg protein (at 25°C and pH 7.5)
Contaminants: (as PK activity = 100%)
Lactate dehydrogenase < 0.02 %
Phosphoglycerate mutase < 0.01 %
Glyceraldehyde-3-phosphate dehydrogenase < 0.01 %
Enolase < 0.01 %
α-Glycerophosphate dehydrogenase < 0.01 %
Myokinase < 0.01 %
State: Purified enzyme in solution
One mL of suspension contains the following
Protein 5 ~ 30 mg
K-PO4 0.1 mol/L
(NH4)2SO4 2.0 ~ 3.0 mol/L
pH 7.0 ± 0.5
Condition for Storage: 2 – 10°C